Wheat Germ Agglutinin as a Potential Therapeutic Agent for Leukemia

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Wheat Germ Agglutinin

Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...

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Tools - Based Search for Potential Human Wheat Germ Agglutinin - Immunoreactive

Following the initial observation about the exsistence of plant lectins-immunoreactive material in different rat and human tissues (D ebb age et al. 1993), we isolated a specific wheat germ agglutinin (WGA)-immunoreactive (IR) protein from human placenta by combination of ion-exchange and immunoaffinity chromatography. It was characterized as 66 kD glycoprotein exhibiting calcium-dependent, car...

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Stoichiometry of Wheat Germ Agglutinin as a Morphology Controlling Agent and as a Morphology Protective Agent for the Human Erythrocyte

The lectin wheat germ agglutinin (WGA) is an unusually effective agent in controlling both the forward and reverse reactions of the reversible morphology conversion discocyte ~:± echinocyte for the human erythrocyte . Under conditions severe enough to drive the reactions to completion in either direction without the lectin, WGA is able to stabilize both these morphologies and to fully prevent c...

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Stoichiometry of wheat germ agglutinin as a morphology controlling agent and as a morphology controlling agent and as a morphology protective agent for the human erythrocyte

The lectin wheat germ agglutinin (WGA) is an unusually effective agent in controlling both the forward and reverse reactions of the reversible morphology conversion discocyte in equilibrium with echinocyte for the human erythrocyte. Under conditions severe enough to drive the reactions to completion in either direction without the lectin, WGA is able to stabilize both these morphologies and to ...

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Immunocytochemical localization of wheat germ agglutinin in wheat

Immunocytological techniques were developed to localize the plant lectin, wheat germ agglutinin (WGA), in the tissues and cells of wheat plants. In a previous study we demonstrated with a radioimmunoassay that the lectin is present in wheat embryos and adult plants both in the roots and at the base of the stem. We have now found, using rhodamine, peroxidase, and ferritin-labeled secondary antib...

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ژورنال

عنوان ژورنال: Frontiers in Oncology

سال: 2019

ISSN: 2234-943X

DOI: 10.3389/fonc.2019.00100